ABSTRACT
Objective: To optimize the ultrasonication method for efficient extraction of P-sitosterol and lupeol from the roots of Astragalus atropilosus using Box-Behnken design of response surface methodology (RSM), and its validation by high performance thin layer chromatography (HPTLC) method. Methods: Ultrasonication method was used to extract β-sitosterol and lupeol from Astragalus atropilosus (roots). RSM was used to optimize the different extraction parameters viz. liquid to solid ratio (10-14 mL/g), temperature (60-80 °C) and time (40-60 min) to maximize the yield of β-sitosterol and lupeol. The quantitative estimation of β-sitosterol and lupeol was done in chloroform extract of Astragalus atropilosus by validated HPTLC method on 10 cm × 20 cm glass-backed silica gel 60F
ABSTRACT
<p><b>OBJECTIVE</b>To develop a simple, selective, sensitive and accurate high-performance thin layer chromatography (HPTLC) method to determine the quantity of hesperidin in different varieties of citrus fruits.</p><p><b>METHODS</b>The method was carried out in aluminum-backed silica gel 60 F254 plates with ethyl acetate-methanol-water 15:3:2 (%, v/v) as mobile phase.</p><p><b>RESULTS</b>A compact band was obtained for hesperidin at Rf value of (0.40±0.04). The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.998 6 was indicative of good linear dependence of peak area on concentration. Limit of detection (8.87 ng/spot), limit of quantification (23.21 ng/spot), accuracy (less than 2%) and recovery (ranging from 98.55-99.38) were found satisfactory.</p><p><b>CONCLUSIONS</b>The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.</p>
ABSTRACT
Ecbalium elaterium, Convolvulus piosellifollus, Cyperus bulbosus and Echinacea angustifolia were selected for studying their hepatoprotective effects against induced liver toxicity by CCI4 in rats. The extent of liver protection is reflected by measuring the biochemical parameters: serum glutamate oxaloacetate transaminase [SGOT], serum glutamate pyruvate transaminase [SGPT], alkaline phosphatase [ALP] and total bilirubin that indicate the condition of liver cells. Extracts that showed good reduction in the biomarker levels were further subjected for histopathological study to explore the extent of protection of hapatocytes. Only the extract of Ecbalium elaterium was effective in reducing the elevated enzyme levels at the 500 mg/kg dose. However, in the histopathological study the extract failed to restore the normal appearance of hepatocytes. All the results were compared with silymarin, as a reference hepatoprotective drug